Figure 3.

 Loss of α-cell character in the Neurog3^Cre-Pdx1^OE pancreas. (A–F) Arx was expressed in Gcg⁺ cells in control in P1 (A) and adult pancreas (C). In B, Arx was found in Gcg⁺, Gcg⁺Ins⁺ (asterisks), and some Ins⁺ cells in P1 Neurog3^Cre-Pdx1^OE (arrows, respectively). Only a few islet mantle Ins⁺ cells expressed Arx in adult Neurog3^Cre-Pdx1^OE (arrows in D and inset). They also expressed Pdx1^HI (arrows in F). (G,H) MafB was detected in Gcg⁺ cells in the control adult pancreas but not Neurog3^Cre-Pdx1^OE. (I,J) Most Ins⁺ cells, except for a few Arx⁺Ins⁺ cells (arrows; also asterisks and separated channels), expressed MafA in Neurog3^Cre-Pdx1^OE adult at levels comparable with control.