Figure 2.

(A) Dose-dependent effects of cannabigivarin (CBGV), Δ⁹-tetrahydrocannabiverin (THCV) and THCV-botanical drug substance (THCV-BDS) on TRPV1-mediated elevation of intracellular calcium in HEK-293 cells stably over-expressing the human recombinant TRP channel of vanilloid type-1 (TRPV1) channel (HEK-293-TRPV1 cells). (B) Dose-dependent inhibitory effects of a 5 min pre-incubation of HEK-293-TRPV1 cells with several pure cannabinoids on the TRPV1-mediated elevation of intracellular calcium induced by capsaicin (0.1 µM). (C) Dose-dependent inhibitory effects of a 5 min pre-incubation of HEK-293-TRPV1 cells with THCV and THCA on the TRPV1-mediated elevation of intracellular calcium induced by capsaicin (0.1 µM). Data are means of at least n = 3 separate experiments. SEM are not shown for the sake of clarity and were never higher than 10% of the means. CBC, cannabichromene; CBD, cannabidiol; CBG, cannabigerol; CBGV, cannabigivarin; CBN, cannabinol; THC, Δ⁹-tetrahydrocannabinol; THCV, propyl analogue of THC or tetrahydrocannabivarin.