Figure 2.

Cannabidiol (CBD) treatment of control, naïve C57BL/6 mice does not affect spinal cord integrity, basal microglial and T-cell number. The Figure shows representative stainings of spinal cord sections prepared from control healthy mice receiving complete Freund adjuvant either without CBD (Ctrl) or with CBD (Ctrl + CBD) treatment. (A) Haematoxylin and eosin (H&E) staining; (B) Iba-1 staining for the presence of microglial/macrophages; and (C) CD3+ staining for presence of T-cells. Scale bars represent 100 µm. Statistical analysis of the CD3+ and Iba-1 expression is presented in the bar graphs of Figures 5C (CD3+) and 6C (Iba-1).