Figure 7.

Cannabidiol (CBD) reduces the increased Mac-2/Galectin-3 expression in the spinal cords of EAE mice. Representative pictures showing that (A) MOG injections increase Mac-2 expression and (B) that CBD injections during the onset of EAE significantly decrease the level of Mac-2 staining. (C) Quantification of Mac-2 fluorescence in spinal cord sections of control and EAE mice with and without CBD treatment. The results are presented as mean ± SEM of total fluorescence from 8–11 spinal fragments from three to four mice per each experimental group. One-way anovaF(3,34) = 63.25, P < 0.0001, followed by Bonferroni post hoc test. *P < 0.001 versus Ctrl. #P < 0.001 versus EAE mice. Scale bar represents 100 µm.