Figure 6.

Phenotypes of disruption of son-1 function by RNAi and overexpression of POP-1. (A–C) Phenotypes observed from son-1 RNAi experiment. (A) Hermaphrodite gonad showing abnormally short gonad arm; (arrow) meiosis at the distal end of the gonad arm. (B) Male gonad (white arrowhead) ectopic germ-line meiosis. (C) gpa-1::lacZ is expressed in two cells per spicule. All four blue cells can be seen on this focal plane, two from the left spicule and two from the right one. Cells from the same side are indicated by linkage of two arrowheads. (D) When POP-1 is overexpressed in wild-type males, gpa-1::lacZ expression is observed in two cells per spicule (black arrowheads). Lateral view, showing only the left spicule. Scale bar, 20 μm.