Figure 8.

Inhibitors of PI3K and mTOR decrease HU210-mediated oligodendrocyte maturation. (A–I) OPC cultures grown for 48 h in the presence of SFM plus PDGF-AA and b-FGF were switched to SFM + T3 and treated for 48 h HU210 (0.5 µM) in the presence or absence of LY294002 (2.5 µM) or rapamycin (1.5 nM). O4 (green) and α-tubulin (red) staining revealed significant arborization of cell processes after HU210 (A–C) that was reversed after LY294002 or rapamycin (D–I). Inhibitors augmented the type A cells, while the mature types B and C populations diminished (**P < 0.01 vs. HU210; *P < 0.05 vs. HU210, one-way anova followed by Tukey's post hoc test) (J).