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. 1997 Oct 15;11(20):2658–2669. doi: 10.1101/gad.11.20.2658

Figure 8.

Figure 8

 Transcriptional activity of TSRE-binding proteins is temperature sensitive in nuclear extracts containing mutant but not wild-type TAFII250. In vitro transcription reactions were carried out in ts13 (A), ts13R3 (B), and HeLa (C) nuclear extracts either in the absence (−) or presence (+) of purified TSRE-binding proteins. After incubation at either 20°C or 30°C, the resulting reaction products were detected by primer extension followed by 8% denaturing PAGE and autoradiography. The reaction products, as a result of accurately initiated transcription from the TSRE–TICAT template DNA (see Materials and Methods for details), are indicated by the arrowheads.

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