Figure 4. KIF3A gene expression of human epithelial RNA from children with uncontrolled asthma and of mouse models with asthma is decreased compared to controls.

Gene expression of KIF3A in nasal epithelial-derived RNA from non-allergic children, controlled and uncontrolled asthmatics was determined by quantitative PCR. GAPDH was used to normalize for expression. To induce an asthma-like phenotype, wild type Balb/c mice were sensitized and challenged with 100 µg of house dust mite (asthma model) and compared to mice sensitized and challenged with phosphate buffered saline (control). One day post-treatment, RNA was extracted from lungs and gene expression measured by quantitative PCR. Actin was used to normalize for gene expression.