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RNase III cleaves pre-U5 to produce intermediates in the absence of other factors. 5′-Labeled or internally-labeled pre-U5 transcripts were incubated for 5 and 45 min with GST–RNT1 or for 45 min with GST alone (Mk). Markers are the phosphorothioate-generated sequence with purines (R) and pyrimidines (Y) as in Fig. 3C.
