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. 2011 Aug 31;6(8):e24099. doi: 10.1371/journal.pone.0024099

Figure 2. Chromatin modulators inhibit cell viability and promote apoptosis in pancreatic cancer stem cells on restoration of miR-34a.

Figure 2

(A), Pancreatic CSCs were treated with SAHA (3 and 5 µM) and 5-Aza-dC (2 and 4 µM) and cell viability was measured at 48 h by staining with trypan blue using Vi-CELL analyzer (Beckman Counter). (B), Pancreatic CSCs were untreated (a) or treated with SAHA (b) or 5Aza-dC (c) for 48 h, and apoptosis was measured by staining with annexin-PI using Accuri Flow Cytometer. (C), Caspase-3/7 activity was measured in pancreatic CSCs treated with SAHA (0.5 and 2 µM) or 5-Aza-dC (1 and 3 µM) for 24 h. Data represent mean ± SD. * and $  =  significantly different from control, P<0.05.