Figure 4.
Experiments confirming the synergistic effect of CCND3 or NRAS with Bcl2 and Myc. (A) In vitro growth curves of various stable integrants in the absence of IL-7 and ST-2 cells. Data are the mean ± s.e.m. (triplicate). (B) Methylcellulose colony-forming abilities of various stable integrants. Data are the mean ± s.e.m. (triplicate). P values are two-sided (Student’s t test). (C) Photograph of representative colonies of various integrants in the first plating. Scale bar represents 10 mm. (D-G) Kaplan-Meier survival curves of SCID mice transplanted with various stable integrants. “3 genes” (n=9) in (D) indicates Bcl2/Myc/CCND3 (n=3), CCND3/Myc/Bcl2 (n=3) and Bcl2/CCND3/Myc (n=3). “2 genes” (n=7) in (D) indicates Bcl2/Myc/mock (n=3), CCND3/Myc/mock (n=2) and Bcl2/CCND3/mock (n=2).