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. 1997 Oct 15;11(20):2679–2690. doi: 10.1101/gad.11.20.2679

Figure 4.

Figure 4

Figure 4

 (A) A truncated DAF-3/GFP protein is predominantly nuclear. Wild-type animals were injected with the truncated construct shown in Fig. 3 at a concentration of 10 ng/ml. The pRF4 transformation marker was injected at 100 ng/ml. The photograph shows an early L2 animal, and DAF-3 is predominantly nuclear. The clear spot in the center of some of the nuclei is the nucleolus. All cells in these animals have predominantly nuclear DAF-3/GFP, including the ventral cord neurons, intestinal cells and distal tip cell (all shown), as well as head and tail neurons and hypodermal cells. (B) DAF-3/GFP is associated with metaphase chromosomes. Fixed L1 animals were immunostained with anti-GFP antibody (Clontech) and anti-α-tubulin antibody (Amersham). DNA was visualized with DAPI.