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. 2011 Aug 31;6(8):e23851. doi: 10.1371/journal.pone.0023851

Figure 3. The changes in DWF4::GUS activity and staining dependent on BR content.

Figure 3

GUS activity was analyzed biochemically (A) and histochemically (B) to examine the effects of Brz and BL on DWF4 transcription. Brz (5 µM) was applied to 14-day-old DWF4::GUS transgenic seedlings and then the seedlings were cultured for 3 days in liquid MS medium to evaluate the effect of Brz, whereas Brz (for 2 days) and BL (0.1 µM; for 1 day) were sequentially applied to evaluate the effect of BL. The graphs show representative results among three independent experiments. GUS assays in each experiment were conducted in triplicate, and the means ± SD were calculated. GUS activity is shown as a value relative to that of the untreated control. The photographs represent the shoots (a–d) and roots (e–h) of transgenic Arabidopsis. The magnification is the same in all photographs, and a bar indicates 1.0 mm (a).