Figure 4. The induction of DWF4::GUS activity by bioactive auxins.

Fourteen-day-old DWF4::GUS transgenic plants were incubated in liquid MS medium containing one of either eight different plant hormones (A), three bioactive auxins or their precursors (B) for 1 or 3 days. GUS activity was measured biochemically. In the graphs, the GUS activity (the mean ± SD) of a representative result among three biological replicates is shown as a value relative to that of the untreated control. BL, brassinolide; IAA, indole-3-acetic acid; GA₃, gibberellic acid; ABA, abscisic acid; BA, benzyl adenine; SA, salicylic acid; ACC, 1-aminocyclopropane-1-carboxylic acid; JA, jasmonic acid; IBA, indole-3-butylic acid; NAA, α-naphtylacetic acid; 2,4-D, 2,4-dichlorophenoxyacetic acid; Trp, l-tryptophan; IAM, indole-3-acetamide.