Figure 2. RT-PCR of putative S-locus genes from stigmatic, stem and leaf tissues using allele-specific forward primers and a universal amplification reverse primer.

(a) Expression of A. munita sequences was detected in stigmatic but not leaf tissue. (b) Expression in P. californicus tissues is highest in stigmas as expected for S-alleles. Actin primers were used as positive controls for all plant tissue cDNA from both species. Putative alleles S2d and S2e appear to be linked. Sequencing of bands in non-stigmatic tissue using S2e primers showed that they are products not previously identified from stigmas. Actin primers were used only once on plant S2 stigmatic tissue, hence there is one less lane in the actin gel than in the allele specific gel.