Table 1.
Number of conversion events in strains homozygous for the RS and MS sequences in the ARG4 gene
| Insert
|
Short conversiona
|
Long conversionb
|
Total number of tetrads analysed
|
||||||
|---|---|---|---|---|---|---|---|---|---|
|
Bst+c conversion
|
Bst−d conversion
|
Total |
Bst+ Age−c coconversion
|
Bst− Age+d coconversion
|
Total | ||||
| number
|
frequency
|
number
|
frequency
|
||||||
| RS | 3 (1) | 4 (2) | 7 (3) | 3% | 7 (1) | 33 (14) | 40 (15) | 21% | 205 |
| MS | 12 (3*) | 12 (3**) | 24 (6) | 11% | 14 (8) | 22 (8**) | 36 (14) | 17% | 215 |
| (38/39) | |||||||||
| MS | 10 (2*) | 12 (3*) | 22 (5) | 12% | 7 (4) | 21 (14) | 30 (18) | 16% | 188 |
| (39/39) | |||||||||
Numbers in brackets correspond to conversion events associated with crossover.
a
Short tract conversion covering only the BstZ17I site (Bst).
b
Long tract conversion encompassing the BstZ17I (Bst) and AgeI (Age) sites including the inserted sequence. The chromosome carrying the URA3 and TRP1 genetic markers and the AgeI restriction site is either the recipientc or the donord in ARG4 gene conversion events.
*
One conversion event is associated with a double crossover.
**
Two conversion events are associated with a double crossover.