Table 3.
Rates of instability and types of alterations for the microsatellite sequences in parental and recombinant spores
Genotype of spores
|
Rate of tract instability
|
Number of spores with additions or deletions of base pairs
|
Total
|
|||
---|---|---|---|---|---|---|
−2
|
0
|
+2
|
+4
|
|||
Parental | 1.3 × 10−2 | 0 | 75 | 1 | 0 | 76 |
Recombinant | 7.7 × 10−2 | 0 | 48* | 3 | 1 | 52 |
Recombinant spores include crossing-over and conversion events encompassing the BstZ17I and/or AgeI restriction sites. The asterix indicates that four of the recombinants classified as microsatellite conversions could be mutants with alterations of the repetitive sequence: Three spores (−2) and one spore (+2).