Figure 1. Ad-CSP Stimulates CS protein specific T and B cell responses.
CS protein specific immune responses increase in an Ad-CSP dose dependent manner. BALB/cJ mice (n = 3) were injected IM with Ad-CSP ranging from 1×107 to 1×109 vps/mouse, increasing by half logs. 14 days post injection splenocytes and plasma were collected. (A) ELISpot assays were performed to quantify IFNγ secreting cells from splenocytes stimulated with CS protein peptide, NYDNAGTNL, ex vivo. (B) IFNγ, TNFα, and IL-2 expression by splenocyte derived CD3+ CD8+ T cells was analyzed by flow cytometry following ex vivo stimulation with NYDNAGTNL. (C) Total IgG against CS protein was assessed by ELISA. The bars represent mean ± SD. Statistical analysis was completed using One Way ANOVA with a Student-Newman-Keuls post-hoc test, *,**,*** denotes significance over naïve, p<0.05, p<0.01, p<0.001.