Figure 4. Loss of localization of apicoplast-targeted proteins in antibiotic-treated, rescued parasites.
Widefield epifluorescence microscopy for apicoplast (ACPL-GFP) and nucleus (Hoescht 33342) comparing ring, trophozoite, and schizont in untreated parasites and chloramphenicol+IPP treated parasites. To right, confocal 3-D reconstruction of untreated parasites and chloramphenicol+IPP treated early trophozoite stage parasites.