FIG. 2.

Suppression of RAGE expression by shRNA increases pancreatic tumor cell sensitivity to oxidative injury. (A) Suppression of RAGE expression increases H₂O₂-induced oxidative cytotoxicity. After transfection with RAGE shRNA or control shRNA for 48 h, Panc2.03 and Panc02 cells were treated with H₂O₂ at indicated dose for 24 h and then the cell viability was analyzed (n = 3, *p < 0.05 versus control shRNA group). A representative Western blot of RAGE level after shRNA is depicted (inset). (B) NF-κB inhibitors increase H₂O₂-induced oxidative injury. Panc2.03 and Panc02 cells were treated with H₂O₂ (0.25 mM) for 24 h with or without NF-κB inhibitors: Bay 11-7085 (“Bay”, 10 μM) and curcumin (50 μM), and the cell viability was analyzed (n = 3, #p < 0.05 versus control shRNA group, *p < 0.05 versus H₂O₂ group).