FIG. 5.

Suppression of RAGE expression decreases activity of NF-κB during oxidative stress. Panc2.03 and Panc02 cells were treated with H₂O₂ (0.25 mM) or gemcitabine (“GEM”, 100 nM) for 24 h. (A) The activity of NF-κB was assayed by luciferase reporter analysis (n = 3, *p < 0.05). (B, C) In parallel, the location of NF-κB p65 in Panc2.03 cells was assayed by confocal microscopic analysis. Representative p65 stains are shown in panel (B), and quantification of the percentage of nuclear p65 positive cells is shown in (C). Data are representative of three experiments (n = 3, *p < 0.05; Bar = 30 μm). Arrows indicate nuclear p65 translocation. (To see this illustration in color the reader is referred to the web version of this article at www.liebertonline.com/ars).