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. 2000 May 15;14(10):1279–1289.

Figure 1.

Figure 1

The bon mutations affect gut tube formation and heart morphogenesis. Light microscopic and immunofluorescent images of wild-type and bon mutant embryos at 30 hpf (A–D) and four days-postfertilization (E,F). (A,B) Compared with wild-type siblings (A), bon mutants (B) show pericardial edema (arrowhead), collapsed brain ventricles (asterisk), an enlarged yolk and a thickened yolk extension (arrow). (C,D) Ventral views of wild-type (C) and bon mutant (D) embryos stained with MF20 (TRITC) and S46 (FITC) antibodies. Red fluorescence indicates MF20 staining of ventricular tissue, whereas yellow fluorescence indicates the overlap of MF20 and S46 staining in atrial tissue. Wild-type embryos have a single heart tube with the ventricle (red) anterior to the atrium (yellow). bon mutants display cardia bifida, with the two separate hearts exhibiting distinct atrial and ventricular tissues. (E,F) bon mutants generally lack a gut tube, although sometimes a small amount of peristaltically contracting gut tissue is present (arrow in F). For comparison, we show a silent heart (sih) mutant where a similar extent of edema allows easy visualization of the gut tube (arrow in E).