Figure 6.

bon overexpression can induce sox17 expression in cyc;sqt double mutants. (A,B) Wild-type (A) and cyc;sqt double mutant (B) embryos were examined for sox17 expression at 90% epiboly. (A–D) Dorsal views. (A,B) sox17 expression is completely absent in cyc;sqt double mutants (B) as determined by genotyping (n = 10). (C,D) Embryos from cyc/+, sqt/+ heterozygote intercrosses were injected with 100 pg of bon mRNA at the 1–4 cell stage, examined for sox17 expression at 75%–80% epiboly and subsequently genotyped. Of 24 cyc;sqt double mutants, no sox17 expression was found in 10 (C), whereas some sox17 expression was observed in 14 (D).