Table 2.
Overexpression of bon mRNA rescues sox17 expression in bon mutants
| Injected mRNA
|
Genotype
|
No. of sox17-expressing endodermal precursors on the dorsal side of the embryo
|
|---|---|---|
| lacZ | wild type | 164.1 ± 5.3 |
| (n = 10) | ||
| lacZ | bonm425 | 35.8 ± 3.0 |
| (n = 20) | ||
| bon | bonm425 | 103.7 ± 4.8 |
| (n = 14) | ||
| lacZ | bons9 | 7.5 ± 0.6 |
| (n = 15) | ||
| bon | bons9 | 80.1 ± 5.4 |
| (n = 16) |
The number of sox17-expressing endodermal precursors was counted at 75%–80% epiboly on the dorsal side of the embryo, which we determined to be representative of the whole embryos. P values (t-test) of the differences between bon and lacZ injections are 0.0001 for both sets of embryos (bonm425 and bons9).