Fig. 3.

Inhibition of AR prevents growth factors-induced expression of adhesion molecules in HT29 cells. (A) The cell surface expression of Vascular cell adhesion molecule, Inter-Cellular Adhesion Molecule and vascular endothelial-cadherin in HT29 cells and HUVEC were analyzed by FACS analysis. (B) The percentage of fluorescent-labeled cells was plotted in a bar diagram. (C) Inhibition of AR prevents growth factors-induced migration of HUVEC in a scratch wound assay. Wound scratches were made with a sterile pipette tip in the wells. After 72 h of incubation, photographs of the wells were taken using Nikon camera fixed to inverted microscope (×100). The line (-) in the figure indicates the level of HUVEC migration. The distance migrated as percentage of total distance was determined for quantitative assessments. Figures are shown from one of the three representative experiments. Bars represent mean ± SEM (n = 4); *P < 0.001 versus control and #P < 0.01 versus growth factor-treated cells.