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. 2011 Oct 15;15(8):2095–2107. doi: 10.1089/ars.2010.3877

FIG. 5.

FIG. 5.

Metabolic failure is prevented by a combination of antioxidants. (A) Redox proteomics experiments were performed in 12 month-old Abcd1− and Abcd1− mice fed for 4 months (Abcd1−+Antx) with NAC and LA. Western blot with an antibody anti-DNP was performed to identify oxidized proteins (n=5/genotype). (B) Western blot (n=4/genotype) against aldolase A (ALDO A), phosphoglycerate kinase (PGK1), pyruvate kinase (PKM2), dihydrolipoamide dehydrogenase (DLD), and mitochondrial aconitase (ACO2). Pyruvate kinase expression (C) and activity (D), NADH, NAD+ levels and the NAD+/NADH ratio (E), NADPH, NADP+ levels and NADP+/NADPH ratio (F), GSH levels, (G) and ATP levels (H) were measured in spinal cord from 12 month-old Wt, Abcd1− and Abcd1− mice fed for 4 months with a cocktail of antioxidants (Abcd1−+Antx) (n=6–7 mice per genotype and condition). Statistical analysis was done with ANOVA followed by Tukey HSD post hoc test. Significant differences are shown as *p<0.05, **p<0.01 and ***p<0.001.