FIG. 1.
Hematopoietic progenitor populations in the bone marrow of Ts65Dn and euploid mice. (A) Hematopoietic progenitor populations as a percentage of nucleated BMC from euploid (open bars) or Ts65Dn (closed bars) mice. Hematopoietic progenitors [long-term reconstituting hematopoietic stem cell (HSC), multipotent progenitor (MPP), and common lymphoid progenitor (CLP) populations] were assessed ex vivo by flow cytometry as defined and described in the Materials and Methods section (n=6, *p<0.05; **p<0.005). (B, C) Competitive repopulation assay was performed as described in the Materials and Methods section with 1:1 mixture of BMC from Ts65Dn mice (closed bars) or euploid littermates (open bars) mixed with an equal number of wild-type BM. BMC repopulating ability was assessed at (B) 3 months and (C) 9 months and is expressed as repopulating units per million BMC. By definition 105 competitor BMC produce 1 repopulating unit (n=4, *p<0.05). BMC, bone marrow cells.