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. 2011 Oct 15;15(8):2083–2094. doi: 10.1089/ars.2010.3798

FIG. 3.

FIG. 3.

Ts65Dn BMC exhibit signs of loss of quiescence. (A) Representative FACS plots of HSC cell cycle analysis using Ki-67 and 7-AAD in Euploid and Ts65Dn mice. (B) Intracellular staining for the Ki-67 antigen was analyzed by flow cytometry as described in the Materials and Methods section. The percentage of cells that are Ki-67(−) were analyzed in total BMC (live gate), lineage-negative (Lin(−)), (Lin−), Sca-1+, c-Kit+ (LSK) population, and the Lin(−), LSK Flk2- (HSC) population. (n=5, *p<0.05). (C) HSC cell cycle analysis by analysis of Ki-67 expression and 7-AAD incorporation showing the percentage of cells in G0, G1, and G2+M phases. (n=4, *p<0.05). 7-AAD, 7-Amino-Actinomycin D.