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. 2011 Oct 15;15(8):2083–2094. doi: 10.1089/ars.2010.3798

FIG. 4.

FIG. 4.

Downregulation of IL-7Rα and Flk2 expression and function in the bone marrow of Ts65Dn mice. (A, B) IL-7Rα expression in selected bone marrow sub-populations was assessed by flow cytometry. (A) Representative FACS plots of IL-7Rα expression in LSK or the Lin(−), SKlo, Flk2+ populations from euploid (solid line) or Ts65Dn (dashed lines) mice. (B) Analysis of the percent cells that are IL-7Rα+ in total BMC (live gate), Lin(−), Lin(−), Sca-1lo,c-Kitlo (SKlo) population, and the Lin(−), SKlo, Flk2+ (Flk2+) population. (n=6, *p<0.05, **p<0.01). (C, D) Flk2 expression in selected bone marrow sub-populations was assessed by flow cytometry. (C) Representative FACS plots of Flk2 expression in LSK cells from euploid (solid line) or Ts65Dn (dashed lines) mice. Marker indicates Flk2-bright population. (D) Analysis of the percent cells that are Flk2+ in total BMC (live gate), Lin(−), Lin(−), Sca-1lo,c-Kitlo (SKlo) population, and the LSK population (n=6, *p<0.05, **p<0.01). (E) Colony formation of Ts65Dn and euploid pre-B cells. Total BMC were cultured in methylcellulose media containing IL-7 and enumerated after 7 days. CFU pre-B: pre-B cell colony forming unit (n=4, *p<0.05). (F) Cell cycle analysis by Ki-67 expression and 7-AAD incorporation of Lin(−) IL-7Rα+ (Lin- IL-7Rα+) lymphoid progenitor cells from Ts65Dn mice (closed bars) or euploid littermates (open bars) (n=4, *p<0.05). (G) Apoptotic cells were detected by AnnexinV binding in selected bone marrow sub-populations from Ts65Dn mice (closed bars) or euploid littermates (open bars). These data are expressed as the percentage of cells in each population that are AnnexinV+ (n=6, *p<0.05).