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. 2011 Oct 15;15(8):2083–2094. doi: 10.1089/ars.2010.3798

FIG. 5.

FIG. 5.

Redox balance affects IL-7Rα expression and B-cell generation in vitro. IL-7Rα (A–C) and B220 (D–F) expression was assessed by flow cytometry in lymphoid progenitor cultures. Lineage-depleted hematopoietic progenitors were cultured in vitro in lymphoid-promoting media as described in the Materials and Methods section. The pro-oxidant BSO (1 mM) and antioxidant NAC (1 mM) were added to selected cultures. IL-7Rα expression was assessed in Lin(−) cells (Lin(−) IL-7Rα+) on day 2 (A), day 5 (B), and day 9 (C). B220 expression was assessed on day 2 (D), day 5 (E), and day 9 (F). The data are expressed as the number of cells in each sub-population (n=3, *p<0.05). BSO, buthionine sulfoximine; NAC, acetylcysteine.