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. 2011 Oct 15;15(8):2083–2094. doi: 10.1089/ars.2010.3798

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Copyright 2011, Mary Ann Liebert, Inc.

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FIG. 6. — Pro/anti-oxidant treatment specifically affects the viability of IL-7Rα-expressing cells. FACS-sorted Lin(−), IL-7Rα⁺ (A) and Lin(−), IL-7Rα (−) (B) hematopoietic progenitors were cultured in vitro in lymphoid-promoting media. The pro-oxidant BSO (0.1 mM) and antioxidant NAC (1 mM) were added to selected cultures and the number of viable cells in each culture condition was assessed using 7-AAD exclusion by flow cytometry on days 2 and 5 (n=2, *p<0.05). (C, D) The percentage of IL-7Rα⁺ cells was assessed in FACS-sorted Lin(−), IL-7Rα⁺ hematopoietic progenitors in the total viable (C) and Sca-1^lo c-Kit^lo Flk2⁺ (D) sub-populations by flow cytometry (n=3, *p<0.05).