FIG. 7.

Oxidative stress in IL-7Rα⁺ BMC both ex vivo and in vitro. (A, B) BMC from euploid (open bars) and Ts65Dn (closed bars) mice were analyzed ex vivo for (A) intracellular GSH in Lin(−), IL-7Rα⁽⁻⁾ or Lin(−), IL-7Rα⁺ cells or (B) PCs in Lin(−), IL-7Rα⁽⁻⁾ (IL-7Rα⁽⁻⁾), Lin(−), IL-7Rα⁺ (IL-7Rα⁺), Lin(−), Sca-1^lo,c-Kit^lo (SKlo), and the Lin(−), SKlo, Flk2⁺ IL-7Rα⁺ (CLP) populations as in Figure 2. (C, D) BMC from wild-type mice were cultured in vitro in lymphoid-promoting media in the absence of any addition (open bars), or in the presence of 0.1 mM BSO (solid bars) or 1 mM NAC (hatched bars) for 2 days as in Figure 5. Cells were analyzed for (C) intracellular GSH or (D) PCs in the indicated subsets as defined in A & B (n=5, *significantly different from euploid or untreated controls, p<0.05; ^#significantly different from IL-7R(−) samples, p<0.05).