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. 2000 May 15;14(10):1196–1208.

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Copyright © 2000, Cold Spring Harbor Laboratory Press

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TAF30 is a component of the NuA3 complex. (A) HAT activity (oligonucleosomes) and TAF30 Western blot analysis of Superose 6 fractions from wild-type and sas3 deletion strains. MonoQ fractions 33–37 from wild-type and sas3 deletion strains were concentrated and put over a Superose 6 sizing column. (Arrows) Fraction in which molecular weight standards elute. (B) Fluorogram of supernatant and beads of immunoprecipitates (IP) of Superose 6 NuA3 fractions using a control Ahc1 antibody (lanes 2,3) or TAF30 antibody (lanes 4,5). I, Input; S, supernatant; B, beads.

TAF30 is a component of the NuA3 complex. (A) HAT activity (oligonucleosomes) and TAF30 Western blot analysis of Superose 6 fractions from wild-type and sas3 deletion strains. MonoQ fractions 33–37 from wild-type and sas3 deletion strains were concentrated and put over a Superose 6 sizing column. (Arrows) Fraction in which molecular weight standards elute. (B) Fluorogram of supernatant and beads of immunoprecipitates (IP) of Superose 6 NuA3 fractions using a control Ahc1 antibody (lanes 2,3) or TAF30 antibody (lanes 4,5). I, Input; S, supernatant; B, beads.