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. 2011 Sep 2;6(9):e23943. doi: 10.1371/journal.pone.0023943

Figure 5. Inhibition of Hh signaling decreases osteopontin and osteopontin-responsive (CD44) positive cells in tumors and peri-tumoral tissues of aged Mdr2−/− mice.

Figure 5

A. Tumor sections from representative DMSO- and GDC-0449 treated Mdr2−/− mice were stained to demonstrate osteopontin (OPN) Representative sections are displayed (Right panel). OPN staining was quantified by morphometric analysis of at least 5 HPF per tumor section using 20× magnification (n = 5 mice/group). Results in the GDC-0449-treated group were normalized to that of the group treated with DMSO vehicle and graphed as fold change. Data are displayed as Mean±SEM (**p<0.01). B. Immunohistochemical staining for the osteopontin receptor, CD44, in peri-tumoral tissues of representative DMSO- and GDC-0449- treated Mdr2−/− mice. (Right panel) CD44 staining was quantified by morphometric analysis as described in A. Results in GDC-0449-treated mice were normalized to those of vehicle-treated controls and graphed as Mean±SEM (**p<0.01). C. QRT-PCR analysis of liver tumor RNA from DMSO- (open bar) and GDC-0449- (closed bar) treated Mdr2−/− mice for OPN (left) and CD44 (right). After normalization to results in the DMSO-treated group, Mean±SEM were graphed (*p<0.05).