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. 2011 Sep 2;6(9):e23148. doi: 10.1371/journal.pone.0023148

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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Confluent monolayers of hfRPE received FITC-labeled POS for 4 hour with or without CNTF (50 ng/ml) and phagocytosis rate were quantified using relative FITC fluorescence intensity normalized to Hoechst 33342 nuclei stain. MFG-E8 (0.6 µM) was used as the positive control. Bar graph A show relative binding+internalization and bar graph B shows internalization only. Bars represent mean ± SEM with triplicate technical repeats. * P<0.05, ** P<0.01, *** P<0.001 compared to negative control. NS: no statistical significance compared to each other. Practically identical results were obtained in another experiment.