Figure 2. Effects of a single amino acid mutation.

Shown here is a Rosetta-generated structure for one mutation (F89I) to yeast calmodulin. The relaxed starting structure is shown in transparent gray, the mutant structure in pink, the native phenylalanine at position 89 in transparent blue, and the mutation to isoleucine in solid red. The mutated structure has accommodated the F89I mutation by small backbone movements, such as the shift in helix position at residue 89, and reconfiguration of nearby side chains.