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. 1998 Mar 15;12(6):880–893. doi: 10.1101/gad.12.6.880

Figure 11.

Figure 11

 Effect of FtsZ overexpression on cell type-specific localization and cell cycle variation of FtsZ. (A) Western blot of FtsZ from swarmer and stalked cells separated by density gradient centrifugation. Loading was normalized for equal number of cells. For NA1000/pZtac, expression of FtsZ was induced with 1 mm IPTG for 2 hr and for NA1000/pUJftsZ FtsZ expression was induced with 0.03% xylose for 1 hr before cell separation. (B) Autoradiograph of immunoprecipitated FtsZ from swarmer and stalked cells of pulse-labeled cultures of NA1000 and of NA1000/pUJftsZ grown with 0.03% xylose for 1 hr. Immunoprecipitation was from equal counts of radiolabeled proteins. (C) Cell cycle Western blot of NA1000/pZtac expressing FtsZ constitutively. ftsZ expression was induced with 1 mm IPTG 1 hr before cell separation and was added to the synchronized culture. Loading was normalized for equal number of cells. (ST) Stalked cells; (SW) swarmer cells.