Figure 3. Cholinergic interneurons exhibit normal response to M₂/M₄, GABA_B and group I mGlu receptor activation.

Representative traces of cholinergic interneurons recorded in the perforated patch-clamp configuration. Recordings were collected from hMT mice at P14 (left) and P90 (right). A–A1. M₂/M₄ receptor activation by oxotremorine (300 nM, 3 min) induced a transient firing cessation with a membrane hyperpolarization. No significant differences were measured at both ages. B–B1. Similarly, application of Baclofen, a GABA_B receptor agonist (10 µM, 2 min) reversibly blocked firing activity in hMT mice. C–C1. Activation of group I mGlu receptors by 3,5-DHPG (10 µM, 30 sec) induced a prominent membrane depolarization coupled with a transient increase of firing rate.