Figure 1.

 Targeted disruption of the Sox1 gene. (A) The structure of the wild-type allele, targeting vector, and targeted allele are shown together with the restriction sites. The open box represents Sox1 coding sequence. The sizes of DNA fragments from the wild-type and mutated allele detected by the 5′ and 3′ probes are indicated. The positions of the PCR primers for genotyping are shown as arrows above the gene. (B) BamHI; (R) EcoRI. (B) Southern blot analysis of BamHI-digested DNA from ES cell clones using 5′ and 3′ probes, (Lanes 1,3) Wild-type ES cells; (lane 2) targeted ES cells. (C) PCR analysis of offspring derived from a mating of mice heterozygous for Sox1 deletion.