Skip to main content
. 2011 Sep 2;6(9):e24486. doi: 10.1371/journal.pone.0024486

Figure 3. Myosin is the target gene for dietary BB effects on bone in OVX animals.

Figure 3

Microarray profiling of bone cell transcripts in RNA from bone with or without BB and OVX. (A), Cluster of myosin related genes and, (B), cluster of collagen turnover related genes. Results are presented as fold changes relative to sham control. Color changes from red to yellow and blue represent decreases in gene expression. (C), Real-time PCR analysis for mRNA of ALP, osteocalcin, Runx2and myosin 2, 4, 7 in bone with different diets and 3 weeks following OVX in female rats. Data are expressed as mean ± SEM (n = 8 per group). Means with different letters differ significantly from each other at p<0.05, a<b<c. (D), Western analysis of myosin and Runx2 in bone from four different diet groups after 3 weeks of OVX, membrane ponceau S staining for protein loading control. Eight to nine samples in each group were randomly pooled into three samples per group are presented for microarray analyses.