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. 2011 Sep 2;6(9):e24486. doi: 10.1371/journal.pone.0024486

Figure 4. BB diet prevents OVX-induced acute bone loss and osteoblastic cell senescence.

Figure 4

(A), Senescence associated beta-galactosidase (SABG) activity analyses in total proteins isolated from bone with four different diet groups. Data are expressed as mean ± SEM (n = 8 per group). Means with different letters differ significantly from each other at p<0.05, a<b<c. (B), Triple staining of immune-staining for myosin and Runx2 and SABG activity staining in long bone sections from four different diet groups 3 weeks after OVX. Pictures are showing typical bone surface osteoblasts from sagittal section under 20 x magnifications, Green stains for myosin, red stains for Runx2 and blue stains for SABG and DAPI staining for nucleus.