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. 1998 Mar 15;12(6):806–819. doi: 10.1101/gad.12.6.806

Figure 2.

Figure 2

Figure 2

 Reduced activation induced cell death in the absence of CPP32. (A,B) [3H]Thymidine uptake and cell death of stimulated CPP32wt and CPP32ex3−/− lymphocytes. (C) [3H]Thymidine uptake of stimulated T cells from rag2−/− chimeric mice reconstituted with CPP32wt and CPP32ex3−/− ES cells. (D) [3H]Thymidine uptake of SEA-stimulated lymphocytes from wild-type and CPP32ex3−/− mice. Purified lymph node responder T cells (1 × 105 cells/well) were activated with plate-bound anti-CD3ε alone or with soluble anti-CD28 mAb or IL-2 (A,C), and SEA (D). After 3, 4, or 5 days of stimulation, [3H]thymidine uptake was determined. Similar experiments were performed with 1 × 106 lymph node cells cultured in 24-well tissue culture plates. At day 4 of stimulation, cells were harvested and the percentage of cell death was determined as indicated in Materials and Methods (B). Each panel shows results representative of at least four independent experiments. Solid bars represent CPP32wt cells; open bars represent CPP32ex3−/− cells.