Figure 8.

 Abnormal chromatin condensation and absence of oligonucleosomal DNA degradation in dying CPP32^ex3−/− 13S/Ras MEFs. (A) Fluorescence microscopy visualization of DAPI staining of DNA from wild-type (+/+), and CPP32^ex3−/− (−/−) MEFs untreated control (Con) or treated with 0.2 μg/ml adriamycin (Adr) and 50 ng/ml mTNF-α for 18 hr and 0.2% serum (LS) for 36 hr. Chromatin condensation in CPP32^ex3−/− did not progress to a fully collapsed state, and no apoptotic bodies were observed on any of the treatments tested. (B) DNA extracted from untreated control (Con) and adriamycin-treated (0.2 μg/ml) wild-type and CPP32^ex3−/− 13S/Ras MEFs. After treatment, wild-type MEFs display the classical laddering degradation of DNA; the laddering does not occur in the CPP32^ex3−/− cells.