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ICP27 is required for the efficient export of late intronless RNAs. HSV-1 KOS or 27–LacZ-infected cells were UV irradiated and immunoprecipitations were performed. The antisense probes used were specific for HSV-1 late intronless mRNAs encoding gD, gC, UL41, and UL15, a late RNA that contains an intron. RNA recovered in the bound fractions (top) represents ∼ 1%–2% of the RNA in the unbound fractions (bottom).
