Figure 11.

 ICP27 binds RNA in vivo through an RGG motif. RSF cells transfected with different ICP27 mutants were infected with 27–LacZ and UV irradiated 6 hr later. RNase protections were performed with a probe specific for the ICP27 transcript. The region spanned by the probe is present in all of the ICP27 mutant RNAs. (A) RNA recovered in the nuclear and cytoplasmic bound fractions. (B) Immunoblot analysis of immunoprecipitated ICP27 protein. Wild-type ICP27 (lanes 1,6,11) migrates at 63 kD. The dark band in all lanes at 55 kD is heavy-chain IgG, which reacts with the secondary antibody and is present because immunoprecipitated proteins were transferred to the membrane.