Figure 9.
ICP27 binds two HSV-1 early intronless mRNAs and facilitates their transport to the cytoplasm. Cells infected with HSV-1 KOS or 27–LacZ for 6 hr were treated with UV light, divided into nuclear and cytoplasmic fractions, and immunoprecipitation was performed. Cells were labeled with [35S]methionine during infection to allow monitoring of immunoprecipitated ICP27 protein (bottom). RNase protection analysis of the bound (top) and unbound (middle) RNA fractions was performed with antisense probes specific for mRNAs encoding TK and ICP8, respectively.