Figure 3. Attenuation of endoplasmic reticulum (ER) stress by dietary wolfberry in db/db type 2 diabetic mice.
After dietary treatments, animals were euthanized. The whole retina was isolated and subjected to Western blot. Representative images were shown (A). β-actin was used as an euqal loading control to normalize each protein expression. Relative band intensity of each protein to β-actin was used for graphing and statistic analysis. n=21. In all cases, the statistic significance at p≤ 0.05 was marked by different letters a,b, and/or c. CD, control diet; WD, control diet with 1 % (kCal) wolfberry. BiP, binding immunoglobulin protein (also named as glucose regulated protein 78, GRP78); PERK, protein kinase RNA-like ER kinase; ATF6, activating transcription factor -6; IB, immunoblot. A. Representative images; B, BiP; C, PERK; D, ATF6; E, active caspase-12; F, active caspase-3.