Table 1.
Genotypic analysis of offspring derived from TRAF6 heterozygous intercrosses
| Stage
|
No. per genotype
|
||||
|---|---|---|---|---|---|
| +/+
|
+/−
|
−/−
|
Total
|
Percentages −/−
|
|
| E14.5 | 5 | 6 | 4 | 15 | 27 |
| E17.5 | 8 | 17 | 7 | 32 | 22 |
| 2 weeks old | 104 | 187 | 36 | 327 | 11 |
Embryos of various stages of gestation were obtained by setting up timed breedings between TRAF6+/− males and females (129 × B6 background). E14.5 was defined as 14.5 days after conception. Viable pups (n = 327) were genotyped ∼2 weeks after birth. Genotypes were determined primarily by PCR analysis. To confirm the validity of the PCR results, genomic Southern blots were performed in parallel for the first several litters.