Table 1.
Neuropeptides identified in the sinus gland (SG) of Callinectes sapidus SG by multiple MS approaches
| Neuropeptide family | m/z | Peptide sequence | Q-TOF | FT | TOF/TOF |
|---|---|---|---|---|---|
| CabTRP | 934.4927 | APSGFLGMRa | + | + | − |
| 950.4921 | APSGFLGM(O)Ra | + | − | − | |
| 980.5027 | TPSGFLGM(O)Ramide | − | + | − | |
| CCAP | 956.3752 | PFCNAFTGCa | + | + | − |
| CPRP[1–9] | 975.5153 | RSAEGLGRMa | + | + | + |
| CPRP[1–10] | 1033.5207 | RSAEGLGRMG | + | + | + |
| CPRP[15–25] | 1216.6896 | SLKSDTVTPLR | + | − | + |
| CPRP[18–28] | 1221.6110 | SDTVTPLRGFE | + | + | − |
| CPRP[14–25] | 1287.7267 | ASLKSDTVTPLR | + | + | + |
| CPRP[1–12] | 1302.7059 | RSAEGLGRMGRL | + | + | + |
| CPRP[13–25] | 1400.8108 | LASLKSDTVTPLR | + | + | − |
| CPRP[1–13] | 1415.7900 | RSAEGLGRMGRLL | + | + | + |
| CPRP[22–36] | 1639.8075 | TPLRGFEGETGHPLE | + | + | − |
| Orcomyotropin | 1168.5422 | FPAFTTGFGSH | + | + | − |
| 1186.5164 | FDAFTTGFGHS | + | + | + | |
| Ork | 1027.4843 | EIDRSGFGF | + | + | − |
| 1066.4800 | NFDEIDRSA | + | + | − | |
| 1098.5214 | EIDRSGFGFA | + | + | − | |
| 1142.5113 | FDEIDRSGFG | + | − | − | |
| 1156.5269 | FDEIDRSGFA | + | + | − | |
| 1186.5375 | FDEIDRSSFA | + | + | − | |
| 1198.5487 | NFDEIDRSGFa | − | + | − | |
| 1199.5327 | NFDEIDRSGF | − | + | + | |
| 1213.5484 | DEIDRSGFGFA | − | + | + | |
| 1228.5593 | NFDEIDRSSFa | + | + | − | |
| 1256.5542 | NFDEIDRSGFG | + | + | + | |
| 1257.5382 | DFDEIDRSGFG | + | + | − | |
| 1270.5699 | NFDEIDRSGFA | + | + | + | |
| 1271.5539 | DFDEIDRSGFA | + | + | − | |
| 1286.5648 | NFDEIDRSSFG | + | + | + | |
| 1287.5488 | DFDEIDRSSFG | + | − | + | |
| 1300.5804 | NFDEIDRSSFA | + | + | − | |
| 1301.5644 | DFDEIDRSSFA | + | + | + | |
| 1360.6168 | FDEIDRSGFGFA | − | + | − | |
| 1403.6226 | NFDEIDRSGFGF | + | + | + | |
| 1433.6332 | NFDEIDRSSFGF | + | + | + | |
| 1474.6597 | NFDEIDRSGFGFA | + | + | + | |
| 1475.6437 | DFDEIDRSGFGFA | + | + | + | |
| 1502.6910 | NFDEIDRSGFGFV | + | + | + | |
| 1503.6750 | DFDEIDRSGFGFV | + | + | + | |
| 1504.6703 | NFDEIDRSSFGFA | + | + | + | |
| 1505.6543 | DFDEIDRSSFGFA | + | + | − | |
| 1532.7016 | NFDEIDRSSFGFV | + | + | + | |
| 1533.6856 | DFDEIDRSSFGFV | + | − | − | |
| 1547.6761 | NFDEIDRSSFGFN | + | + | + | |
| 1548.6601 | DFDEIDRSSFGFN | + | − | + | |
| 1554.6972 | NFDEIDRTGFGFH | − | + | + | |
| Ork/Orcomyotropin-related | 1062.5578 | TPRDIANLY | + | − | − |
| Others | 844.4788 | HLGSLYRa | − | + | + |
| 902.5458 | KIFEPLR | − | + | − | |
| 1259.7106 | KIFEPLRDKN | − | − | + | |
| PDH | 1190.5762 | pQELHVPEREA | + | − | − |
| 1927.1800 | NSELINSILGLPKVMNDAa | + | − | − | |
| proctolin | 649.3668 | RYLPT | − | + | + |
| RFa | 925.4890 | ETNFLRFa | − | − | + |
| 1005.5741 | GRPNFLRFa | − | + | − | |
| 1029.5629 | DHVPFLRFa | − | + | − | |
| 1045.5802 | GHRNFLRFa | − | − | + | |
| 1059.5959 | AHRNFLRFa | − | + | − | |
| 1104.6061 | GAHKNYLRFa | + | + | + | |
| 1116.5473 | YEQDFLRFa | − | + | − | |
| 1146.6055 | GYSKNYLRFa | − | + | + | |
| 1172.6323 | AYNRSFLRFa | − | + | + | |
| 1174.6368 | FTSKNYLRFa | − | − | + | |
| 1209.6123 | DENRNFLRFa | − | + | − | |
| 1273.6436 | YGSDRNFLRFa | − | − | + | |
| 1314.7753 | DARTAPLRLRFa | − | + | − | |
| RPCH | 930.3443 | pELNFSPGWa | + | − | − |
| RYa | 1030.4703 | pEGFYSQRYa | + | − | + |
| SIFa | 1381.7375 | GYRKPPFNGSIFa | − | + | − |
Peptides identified by de novo sequencing using ESI-Q-TOF, MALDI-FTMS and MALDI-TOF/TOF. Neuropeptides listed in red are novel peptides sequenced for the first time in this study. Lower case “a” at the C-terminal peptide indicates C-terminal amidation. CabTRP: Cancer borealis tachykinin-related peptide; CCAP: Crustacean cardioactive peptide; CPRP: CHH precursor-related peptide; PDH: pigment dispersing hormone; RPCH: red pigment concentrating hormone; Ork: Orcokinin