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. Author manuscript; available in PMC: 2012 Sep 1.
Published in final edited form as: J Neurochem. 2011 Aug 8;118(6):1087–1100. doi: 10.1111/j.1471-4159.2011.07386.x

Fig. 2. Wild-type THAP1 binds itself and most DYT6 mutants, but not Q154fs180X.

Fig. 2

(A) Representative immunoblot demonstrating coimmunoprecipitation of wild-type THAP1-FLAG (wtTHAP-FLAG) by different V5-tagged THAP1 variants in lysates from transfected HEK-293T cells. No wtTHAP1-FLAG was immunoprecipitated by anti-V5 from control lysates expressing wtTHAP1-FLAG alone. All of the V5-tagged THAP1 variants coimmunoprecipitated wtTHAP1-FLAG except for the Q154fs180X mutant, which lost the interaction. (B) Relative differences in levels of wtTHAP1-FLAG immunoprecipitated by each V5-tagged THAP1 variant. Data for each DYT6 mutant are expressed as mean fold changes relative to V5-wtTHAP1, +/− standard errors from four independent experiments. Normalization of densitometry values is described in Materials and Methods.